PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

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121 – 135 of 8,322 results

121

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)₁₈ Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase	M-MLV
Size (Final Product)	7 kb or less
Final Product Type	cDNA
Sample Type	Mammalian Cells
Purification Time	2 hr.
Optimal Reaction Temperature	42°C
For Use With (Application)	Real Time PCR (qPCR),RT-PCR
Product Line	Ambion, Cells-to-cDNA

122

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Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

The Ambion Single Cell-to-C_T™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Purification Time	7 min.
Starting Material	1-10 Cells
For Use With (Application)	RT-PCR
PCR Method	2-step RT-qPCR
Product Line	Ambion, Cells-to-CT
Detection Method	TaqMan

123

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Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

Pricing & Availability
Specifications

Polymerase	AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	Multiplex Master Mix
Sample Type	DNA
Reaction Speed	Fast
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	Multiplex qPCR
Product Line	TaqMan
Detection Method	Primer-probe

124

Thermo Scientific™ dNTP/dUTP Mix

Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM.

Pricing & Availability

125

Thermo Scientific™ Aminoallyl-dUTP Solution (10 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Pricing & Availability

126

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Invitrogen™ AccuPrime™ Taq DNA Polymerase System

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	2X
Overhang	3'-A
Concentration	10X
For Use With (Application)	Hot-start PCR
PCR Method	1-step RT-qPCR,Multiplex qPCR
Detection Method	Primer-probe
Polymerase	AccuPrime Taq DNA Polymerase
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase System
Reaction Speed	Standard
Hot Start	Built-In Hot Start

127

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Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq DNA Polymerase
Format	One-Step RT-PCR System
Size (Final Product)	Up to 4.5 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

128

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Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum SuperFi U DNA Polymerase
GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 10 kb
Final Product Type	PCR Amplified cDNA
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription, Preamplification
Starting Material	Cells or RNA
For Use With (Application)	Next-Generation Sequencing (NGS),Real Time PCR (qPCR)

129

Thermo Scientific™ dNTP Mix (20 mM)

Thermo Scientific dNTP Mix (20 mM) contains purified deoxyribonucleotide triphosphates (dNTPs) that meet high quality standards that result in excellent performance for a variety of molecular biology applications.

Pricing & Availability

130

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Invitrogen™ SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
Concentration	5X
For Use With (Application)	Real Time PCR (qPCR)
Detection Method	qPCR
Format	Master Mix
Size (Final Product)	Up to 10 kb
Reaction Format	Master Mix
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

131

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Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	AccuPrime Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	9X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	Built-In Hot Start
For Use With (Application)	Hot-start PCR,High-fidelity PCR
Detection Method	Primer-probe

132

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Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.

Pricing & Availability

133

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Invitrogen™ AccuPrime™ SuperMix I

AccuPrime SuperMix I is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Pricing & Availability

134

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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	AccuPrime Pfx DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	26X
Size (Final Product)	12 kb or less
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR,High-fidelity PCR
Detection Method	Primer-probe

135

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Applied Biosystems™ TaqMan™ GTXpress™ Master Mix

Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR

PCR Equipment and Supplies

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Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ TaqMan™ Multiplex Master Mix GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase